Isolate and sequence ribosome-protected mRNA fragments using size-exclusion chromatography

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Isolate and sequence ribosome-protected mRNA fragments using size-exclusion chromatography

Investigating translationally active mRNA involves isolating and sequencing RNA directly associated with the ribosome. Traditionally, isolating these complexes involved sucrose gradient ultracentrifugation, which is tedious and technically difficult. ARTseqTM (active RNA translation) Ribosome Profiling Kits provide an alternative method for obtaining ribosome-protected RNA fragments using size-...

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Ribosome profiling identifies ribosome positions on translated mRNAs. A prominent feature of published datasets is the near complete absence of ribosomes in 3' untranslated regions (3'UTR) although substantial ribosome density can be observed on non-coding RNAs. Here we perform ribosome profiling in cultured Drosophila and human cells and show that different features of translation are revealed...

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Size-exclusion chromatography using core-shell particles.

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MOTIVATION Ribosome profiling is a new technique that allows monitoring locations of translating ribosomes on mRNA at a whole transcriptome level. A recent ribosome profiling study demonstrated that internal Shine-Dalgarno (SD) sequences have a major global effect on translation rates in bacteria: ribosomes pause at SD sites in mRNA. Therefore, it is important to understand how SD sites effect ...

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ژورنال

عنوان ژورنال: Nature Methods

سال: 2013

ISSN: 1548-7091,1548-7105

DOI: 10.1038/nmeth.f.363